1. Make blacklist for ADF containing all barcodes (1 per line) that are doublet class 2 2. Make UMAP file 3 column. Barcode,x,y 3. Make Cluster files, leiden clustering for all resolutions fro SCbestpractices. 4. Make feature selection list for both Nodule_1 and Nodule_2. 5. Do integration with Seurat now that you are using the correct data.